Description
A Diluting Pipette is a tool used in to dispense a measurable quantity of blood. Various pipettes are used in hematology which includes RBC & WBC pipettes, Hemoglobin pipette etc. Here we will understand the differences between RBC & WBC pipettes and their various uses.
1. What is the other name of RBC/WBC pipette used to make dilutions of whole blood?
Thoma pipette
2. Describe the R-B-C Pipette
RBC pipette has red bead in the bulb and it has marking of 0.5, 1 and 101.
3. What is the role of red bead in pipette?
Helps in identification of pipette
Helps in mixing of diluting fluid and blood
To ensure dryness of pipette: Free mobility indicates a dry pipette
4. What are the other uses of RBC pipette?
It is used wherever higher dilution is required Eg; High total leukocyte count and total sperm count
5. Name the diluting fluid used for RBC count
Hayem’s fluid, Dacie’s fluid
6. What is the dilution used in RBC pipette?
1:200
7. What is the volume of blood the pipette delivers
It delievers 20ul or 0.02 ml of blood.
8. What is the composition of Hayem’s fluid
Hayem’s fluid: a. Sodium chloride -0.5 gm
b. Sodium sulfate -2.5 gm
c. Mercuric chloride -0.25 gm
d. Distilled water – 100 ml.
9. What is the composition of Dacie’s fluid:
Dacie’s fluid:
a. Trisodium citrate – 3 gm.
b. Formalin – 1 ml.
b. Distill water – 99 ml.
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